Enzymes & cloning

Virtual gel

Predict restriction-digest fragment sizes and their gel migration positions against a chosen DNA ladder.

Batchable via POST /batch.

post/virtual_gel

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/virtual_gel
  • Auth: none required

Request body

sequencestring required

Nucleotide sequence (raw or FASTA; IUPAC accepted).

enzymesstring[]

Enzyme names to digest with, from the curated common-enzyme set (see restriction_sites for the full list). An unrecognized name is rejected rather than skipped, so an empty band pattern always means "no sites".

circularboolean

Treat the sequence as circular (plasmid).

ladder'1 kb' | 'GeneRuler 1 kb' | '1 kb Plus' | '1 kb Plus (legacy)' | '100 bp'

DNA ladder to plot alongside the sample lane.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.