Enzymes & cloning
Assemble a multi-gene operon and check it
Build a polycistronic operon from a promoter, a list of CDSs with their RBSs, spacers and a terminator — optionally recoding every CDS for a host — then scan the ASSEMBLED molecule for internal promoters, Shine-Dalgarno sequences, terminators, out-of-frame start codons and repeats. Scanning the product rather than the parts is the point: these elements are very often created BY THE JOIN between two parts, so checking each part alone finds nothing. Returns the sequence, an annotated GenBank file, and every element found. Assigns no translation rate — use rbs_library_design to choose RBSs, then rbs_predict to rank the result.
post/operon_design
Request
- Base URL: https://seqbench.com/api/v1
- URL: https://seqbench.com/api/v1/operon_design
- Auth: none required
Request body
Response
Tool ran successfully.
Example response
{
"provenance": {
"apiVersion": "1.1.0"
}
}Changes
No recorded changes to this operation across all 1 revision of this API.