Enzymes & cloning
Diagnostic digest planner
Pick the restriction digest that tells your intended construct apart from the wrong ones on a screening gel. Digests every candidate, works out which bands would actually resolve at the chosen agarose percentage (size ratio, the gel's resolving window, and whether a band is too faint to score), and ranks single enzymes — then buffer-checked pairs if no single one works. The criterion is separating the INTENDED construct from every alternative; telling the alternatives apart from each other is reported as a bonus. Get the alternatives from assembly_outcomes.
post/diagnostic_digest
Request
- Base URL: https://seqbench.com/api/v1
- URL: https://seqbench.com/api/v1/diagnostic_digest
- Auth: none required
Request body
Response
Tool ran successfully.
Example response
{
"provenance": {
"apiVersion": "1.1.0"
}
}Changes
No recorded changes to this operation across all 1 revision of this API.