Enzymes & cloning

Golden Gate overhang fidelity

Score a candidate set of 4-base Golden Gate/MoClo junction overhangs against real published T4-ligase ligation-count data: per-overhang specificity, the weakest link in the set, and any risky cross-reacting pairs. Optionally compare against a named published overhang set. This is SeqBench's own transparent scoring methodology — it does not reproduce NEB's/Potapov's own published aggregate fidelity percentages for named sets (their exact formula isn't disclosed anywhere accessible).

post/golden_gate_fidelity

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/golden_gate_fidelity
  • Auth: none required

Request body

overhangsstring[] required

The candidate 4-base overhangs for one assembly (e.g. ["GGAG","TACT","AATG"]). At least 2, no duplicates.

dataset'generic-t4-37c-1h' | 'bsai-hfv2'

Which real ligation dataset to score against — generic T4 ligase, or an enzyme-specific one-pot dataset if that matches your actual digestion enzyme.

riskThresholdnumber

Flag a pair as risky when the cross-reaction is at least this fraction of that pair's own total signal.

compareToNamedSet'pryor-2020-plant-11' | 'pryor-2020-20set' | 'cidar-moclo'

Also score this published reference set (see namedSetsAvailable in the output) alongside your candidate set, for comparison.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.