Analysis

qPCR ΔΔCt fold change

Relative quantification from Ct values: ΔCt, ΔΔCt and fold change, with the standard error propagated from replicates. Picks the method from the amplicon efficiencies rather than leaving it to you — Livak's 2^-ΔΔCt when both are 100% efficient, the Pfaffl efficiency-corrected ratio when they are not, since running Livak over amplicons that do not qualify is the failure this tool exists to prevent. Reports what Livak would have said, so the size of that difference is visible.

post/qpcr_ddct

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/qpcr_ddct
  • Auth: none required

Request body

sampleTargetnumber[] required

Ct values for the gene of interest in the treated/test sample. Replicates as a list.

sampleReferencenumber[] required

Ct values for the reference (housekeeping) gene in the same sample.

controlTargetnumber[] required

Ct values for the gene of interest in the calibrator/control sample.

controlReferencenumber[] required

Ct values for the reference gene in the control sample.

targetEfficiencynumber

Per-cycle amplification fold for the target amplicon. 2.0 = 100%. From a standard curve, E = 10^(-1/slope).

referenceEfficiencynumber

Per-cycle amplification fold for the reference amplicon. 2.0 = 100%.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.