Analysis

OD600 to cells/mL

Convert an OD600 reading to a cell density and a total cell count, correcting for the optical path and any dilution, and flagging the two things that silently make the number wrong: a reading above the linear range (a dense culture reads LOW), and a plate-reader path length assumed to be a cuvette's 1 cm. Also plans a back-dilution to a target OD. The cells-per-OD factor is a convention, not a constant — it varies with strain, growth phase and instrument, so pass factorPerOd when you have calibrated against plate counts.

post/od600_cells

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/od600_cells
  • Auth: none required

Request body

od600number required

The reading off the spectrophotometer.

organism'ecoli' | 'scerevisiae' | 'ppastoris'

Supplies the cells-per-OD factor. Ignored when factorPerOd is given.

factorPerOdnumber

Your own calibration: cells/mL at OD600 = 1.0 in a 1 cm path. Overrides organism.

pathLengthCmnumber

Optical path of the reading. A cuvette is 1 cm; 200 µL in a 96-well flat plate is about 0.58 cm.

dilutionFactornumber

Dilution done before reading (2 means the sample was diluted 1:2).

volumeMlnumber

Culture volume, for a total cell count. 0 = not given.

targetOdnumber

Optional: plan a back-dilution to this OD. 0 = skip.

targetVolumeMlnumber

Final volume for the back-dilution plan.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.