Analysis

Sigma-70 promoter strength

Scan DNA for E. coli sigma-70 promoters and estimate each one's transcription initiation rate, with the free-energy terms it is built from: the -35 and -10 boxes, the spacer, the discriminator, the extended -10 and the initial transcribed region. Pairs with rbs_predict — together they separate 'nothing is transcribed' from 'it is transcribed and not translated', which no single measurement on the sequence does. Both strands by default, because a promoter reading into your insert from the other strand is still a promoter.

post/promoter_predict

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/promoter_predict
  • Auth: none required

Request body

sequencestring required

DNA to scan, 80-30000 nt. A promoter region, a 5' UTR with its upstream context, a whole cassette, or a plasmid (past 10000 nt it is scanned in overlapping windows).

bothStrandsboolean

Scan the reverse strand too. Leave on unless you know the orientation — cryptic antisense promoters are a common and easily missed cause of trouble.

limitnumber

How many of the strongest promoters to return.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.