Check an oligo order against its map
Check a list of oligos against the plasmid or template they were designed from, before ordering them. For each oligo: where it primes (3'-anchored, so cloning tails are allowed), on which strand, and which bases disagree with the map. When an oligo primes nowhere it says WHICH copying mistake explains it — reversed without being complemented, complemented without being reversed, one base dropped or doubled, a wrong last base — and gives the sequence that was meant, but only when exactly one such mistake reproduces an oligo that binds. It simulates every product the order can make (up to 15,000 bp) with the in_silico_pcr engine, and for pairs the names declare (GFP_F / GFP_R) says why they make none, including which of the two is written as the wrong strand. Also flags one sequence under two names, one name on two sequences, a primer saved in the map under the same name that sits elsewhere, and 3' self-dimers/hairpins. Up to 96 oligos.
Request
- Base URL: https://seqbench.com/api/v1
- URL: https://seqbench.com/api/v1/oligo_order_check
- Auth: none required
Request body
Response
Tool ran successfully.
Example response
{
"provenance": {
"apiVersion": "1.1.0"
}
}