Primers & oligos

ASO Gapmer Designer

Design antisense-oligonucleotide (ASO) gapmers against an mRNA target: scans candidate sites, builds the antisense oligo in the standard 5-10-5 architecture (chemically-modified wings, central DNA gap for RNase H1, phosphorothioate backbone), and screens each for known liabilities (G-quadruplex motifs, CpG immunostimulation, self-complementarity, GC extremes). No transcriptome-wide off-target search.

post/aso_design

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/aso_design
  • Auth: none required

Request body

targetstring required

Nucleotide sequence (raw or FASTA; IUPAC accepted).

lengthinteger

Total gapmer length (nt). A real RNase H1 gapmer is 12-25.

winginteger

Modified-wing length on each side (nt); the central gap = length − 2×wing, so 2×wing must be less than length.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.