Primers & oligos

Primer Tm calculator

Primer/oligo melting temperature: nearest-neighbor (SantaLucia 1998) at the supplied reaction conditions, recommended from 14 nt up, with the Wallace rule for shorter oligos, a fixed-100 mM-Na+ Schildkraut-Lifson reference estimate, and molecular weights.

Batchable via POST /batch.

post/melting_temperature

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/melting_temperature
  • Auth: none required

Request body

sequencestring required

Nucleotide sequence (raw or FASTA; IUPAC accepted, and "I" for deoxyinosine).

oligoNMnumber

Total strand concentration (nM).

naMMnumber

Monovalent cation [Na+]/[K+] (mM).

mgMMnumber

Divalent cation [Mg2+] (mM).

dntpMMnumber

Total [dNTP] (mM), chelates Mg2+.

targetTmnumber

Optional target Tm (°C). When given, the response includes a pass/fail gate.

tmTolerancenumber

Allowed +/- window (°C) around targetTm for the gate.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.