Primers & oligos

Oligo analyzer

Full oligo analysis: nearest-neighbor Tm/ΔG/ΔH/ΔS plus hairpin and self-dimer screening with base-pair diagrams and warnings.

Batchable via POST /batch.

post/oligo_analysis

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/oligo_analysis
  • Auth: none required

Request body

sequencestring required

Nucleotide sequence (raw or FASTA; IUPAC accepted).

oligoNMnumber

Total strand concentration (nM).

naMMnumber

Monovalent cation [Na+]/[K+] (mM).

mgMMnumber

Divalent cation [Mg2+] (mM).

dntpMMnumber

Total [dNTP] (mM), chelates Mg2+.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.