Files & formats

Sanger vs Reference

Align a Sanger ABIF read to a reference and report identity plus every mismatch, insertion and deletion.

Batchable via POST /batch.

post/sanger_vs_reference

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/sanger_vs_reference
  • Auth: none required

Request body

fileBase64string

The binary ABIF (.ab1 / .abi) trace file, base64-encoded.

readstring

Sanger read as FASTA or raw text (alternative to uploading an ABIF trace). Also the per-record field for plate-batch runs via /api/v1/batch.

referencestring required

Expected reference sequence (FASTA or raw).

fileNamestring

Optional original file name (echoed back).

minCoveragenumber

Fraction of the reference the read must span before a PASS is meaningful; below this the verdict is 'ambiguous_low_coverage' regardless of identity. Lower it when the reference is intentionally just the region/junction being checked.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.