Proteins & peptides

Protein Properties

Protein properties: molecular weight, isoelectric point, GRAVY, extinction coefficient and composition.

Batchable via POST /batch.

post/protein_properties

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/protein_properties
  • Auth: none required

Request body

sequencestring required

Protein sequence (one-letter amino-acid codes; non-AA characters ignored).

chargeStepnumber

pH step along the net-charge titration curve, which always spans pH 0-14. Between 0.001 and 14.

a280number

A measured absorbance at 280 nm. When given, the response adds concentration in mg/mL and µM for both extinction coefficients.

pathLengthCmnumber

Path length of the A280 reading in cm (a NanoDrop already reports values normalised to 1 cm).

dilutionnumber

How many-fold the sample was diluted before the A280 was read.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.