Proteins & peptides

Protease Digestion

In-silico protease/chemical digestion: cleave a protein and report each peptide's position, length and neutral mass. CNBr masses assume terminal Met becomes homoserine lactone; the sequence retains M and the response labels the modification.

Batchable via POST /batch.

post/protease_digestion

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/protease_digestion
  • Auth: none required

Request body

sequencestring required

Protein sequence (one-letter amino-acid codes; non-AA characters ignored).

protease'trypsin' | 'lysc' | 'argc' | 'chymotrypsin' | 'gluc' | 'aspn' | 'cnbr'

Protease or chemical cleavage agent.

missedCleavages0 | 1 | 2

Allowed missed internal cleavages (0–2).

minMassnumber

Optional lower bound on neutral monoisotopic mass (Da).

maxMassnumber

Optional upper bound on neutral monoisotopic mass (Da).

maxPeptidesinteger

Cap on the number of returned peptides.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.