CRISPR

twinPE Studio (dual pegRNA)

Design a twinPE pegRNA pair (Anzalone et al. 2022) for a replacement too large for a single pegRNA's RTT: a left pegRNA nicks the + strand at/before the replacement window and a right pegRNA nicks the - strand at/after it, each synthesizing a new 3' flap; both flaps are truncated at a shared overlap in the middle of the new sequence so they anneal and resolve the edit without an HDR donor. Coordinates: both pegRNAs' protospacerStart/protospacerEnd/nickPosition are 1-based inclusive in the submitted PRE-EDIT target's frame (the PAM search runs on the unedited sequence, on both sides), while replaceSpan is the span of the new content in the returned editedSequence. Off-target activity is not evaluated (no in-browser reference genome).

post/prime_editing_twin_design

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/prime_editing_twin_design
  • Auth: none required

Request body

targetstring required

Forward-strand target DNA (raw or FASTA), with flanking sequence on both sides of the replacement window.

replaceStartinteger required

1-based inclusive start of the region being replaced/deleted.

replaceEndinteger required

1-based inclusive end of the region being replaced/deleted. For a pure insertion, set replaceEnd = replaceStart - 1.

newSequencestring required

New sequence (forward strand) to install in place of [replaceStart, replaceEnd]. Needs >= 2 bp.

overlapLengthinteger

Length (bp) of the shared overlap built into both pegRNAs' 3' flaps where they meet and anneal.

pbsLengthinteger

Optional preferred PBS length to highlight; a full 8-17 nt sweep is always returned.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.