CRISPR

Base Editing Designer

Design cytosine (CBE, C→T) or adenine (ABE, A→G) base-editing gRNAs for an SpCas9 target: for each NGG gRNA it reports every editable base inside the editor's activity window, flags bystander edits (more than one editable base in the window), and — with a CDS reading frame — classifies each edit's amino-acid consequence (silent / missense / nonsense / stop-loss). Bystander-free guides are ranked first. Handles both strands (a C→T on the protospacer of a reverse-strand guide is reported as the forward-strand G→A).

post/base_editing_design

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/base_editing_design
  • Auth: none required

Request body

targetstring required

Nucleotide sequence (raw or FASTA; IUPAC accepted).

editor'be3' | 'be4max' | 'abe7.10' | 'abe8e'

Base editor: be3/be4max (CBE, C→T) or abe7.10/abe8e (ABE, A→G).

frameStartinteger

Optional 1-based CDS reading-frame start, to classify each edit's amino-acid consequence.

targetPositioninteger

Optional 1-based forward-strand position of the base you intend to edit; only guides whose window covers it are returned.

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.