CRISPR

CRISPR gRNA designer

Find and score candidate guide RNAs (protospacer + PAM) in a target DNA for common nucleases (SpCas9, SpCas9-NG, SaCas9, Cas12a).

Batchable via POST /batch.

post/crispr_grna_design

Request

  • Base URL: https://seqbench.com/api/v1
  • URL: https://seqbench.com/api/v1/crispr_grna_design
  • Auth: none required

Request body

sequencestring required

Nucleotide sequence (raw or FASTA; IUPAC accepted).

nuclease'spcas9' | 'spcas9ng' | 'sacas9' | 'cas12a'

Nuclease id. Omit to just list the available nucleases (no scan is performed).

searchReverseStrandboolean

Also scan the reverse strand for guides.

minScorenumber

Only return guides with a heuristic score at least this high (0–100).

Response

Tool ran successfully.

okunknown required
toolstring required

The tool slug that ran.

resultobject required

Tool-specific output object. Its shape depends on the tool — call GET /{tool} or see the docs for each tool's fields.

Example response

{
  "provenance": {
    "apiVersion": "1.1.0"
  }
}

Changes

No recorded changes to this operation across all 1 revision of this API.