BEbenchling
dnaSequences

Create codon-optimized DNA sequences.

Create codon-optimized DNA sequences.

post/dna-sequences:optimize-codons

Request

  • Base URL
    https://{tenant_name}.benchling.com/api/v2
  • Auth
    HTTP bearer

Request body

avoidedCutsiteEnzymeIdsstring[]

List of enzyme IDs whose recognition sites will be avoided when creating the optimized sequence.

codonUsageTableIdstring

ID of the codon usage table representing the target organism.

dnaSequenceIdsstring[] required

IDs of DNA sequences to codon-optimize.

folderIdstring required

ID of the folder in which the optimized sequences will be saved.

gcContent'ANY' | 'LOW' | 'MEDIUM' | 'HIGH'

The amount of GC content in the optimized sequence. LOW is defined as below 0.33, MEDIUM as 0.33-0.66, and HIGH as above 0.66. If neither gcContent nor gcContentRange is specified, the optimization will default to ANY (0-1). Cannot be specified together with gcContentRange.

method'MATCH_CODON_USAGE' | 'USE_BEST_CODON'

The codon optimization algorithm to use. Requires codonUsageTableId to be specified. MATCH_CODON_USAGE selects codons probabilistically based on the organism's codon usage frequencies. USE_BEST_CODON always selects the most frequently used codon for each amino acid.

schemaIdstring

ID of the optimized DNA sequences' schemas

shouldDepleteUridineboolean

If not specified, the optimization will default to false, and mRNA uridine depletion will not be performed.

Response

This endpoint launches a long-running task and returns the Task ID of the launched task. When successful, the task has an empty response.

taskIdstring

Changes

No recorded changes to this operation across all 1 revision of this API.