---
title: "Verify a claimed construct"
method: POST
path: "/verify_construct"
tags: ["Enzymes & cloning"]
---

# Verify a claimed construct

`POST /verify_construct`

## Request

- Base URL: `https://seqbench.com/api/v1`
- URL: `https://seqbench.com/api/v1/verify_construct`
- Auth: none required

Re-derive a construct's insert from the PCR (template + primers) claimed to have produced it, then check — independently of that claim — whether the expected insert actually appears (either orientation) in the claimed final construct, at what identity, and with exact mismatch positions if not. Optionally also checks for a premature stop in a declared reading frame. Primers may carry a non-templated 5' tail (a restriction site, a Gibson arm, a tag): a construct missing ONLY tail bases still passes, since that is exactly what digesting a tailed amplicon removes before ligation — see match.templateCoveragePct and match.unalignedIsTailOnly, and note the pass does not establish that the right enzyme made the cut. This re-derives from the claim's own stated inputs; it does not review the claim's prose.

## Request body

- object
  - `claimedConstruct` string, required: The final sequence claimed to have been built.
  - `insertTemplate` string, required: PCR template the insert was amplified from.
  - `insertForwardPrimer` string, required: Forward primer used to amplify the insert, 5'→3'.
  - `insertReversePrimer` string, required: Reverse primer used to amplify the insert, 5'→3'.
  - `templateCircular` boolean: Treat insertTemplate as circular (e.g. amplifying from a plasmid).
  - `constructCircular` boolean: Treat claimedConstruct as a circular plasmid, so an insert that spans its arbitrary numbering origin is found whole. Without it the insert is looked for linearly and a wrapped one covers only its longer half.
  - `maxPrimerMismatches` integer: Mismatches tolerated per primer during PCR prediction (0–10).
  - `expectedFrameStart` integer: 1-based position in claimedConstruct where the intended reading frame begins. If given, flags a premature stop before the end of the aligned insert region.

## Response `200`

Tool ran successfully.

- SuccessResponse: Standard success envelope returned by every single-tool call.
  - `ok` unknown, required
  - `tool` string, required: The tool slug that ran.
  - `result` object, required: Tool-specific output object. Its shape depends on the tool — call `GET /{tool}` or see the docs for each tool's fields.
  - `gate` GateResult
    - `pass` boolean, required: True if and only if every hard check passed.
    - `checks` GateCheck[], required
      - `id` string, required
      - `label` string, required
      - `severity` 'hard' | 'soft', required
      - `pass` boolean, required
      - `message` string, required
      - `value` number
      - `threshold` string
    - `notChecked` string[], required: Honest list of what this gate does NOT verify.
  - `provenance` Provenance, required
    - `apiVersion` string, required
    - `tool` union, required: Tool slug, or an array of slugs (one per step) for a workflow.
      - string
      - string[]
    - `generatedAt` string, date-time, required: ISO 8601 timestamp of when the result was generated.

## Other responses

- `400`: Invalid argument or the tool could not run.
- `404`: Unknown tool.
- `429`: Rate limited (metered tools only). Body carries code `rate_limited` and retryable `true`; honor the Retry-After header.
- `503`: A metered tool is momentarily at capacity. The identical request should succeed after Retry-After; body carries code `upstream_unavailable`.

---

[API](https://skmtc.dev/seqbench/apis/seqbench-api.md) · [All operations](https://skmtc.dev/seqbench/apis/seqbench-api/llms.txt) · [OpenAPI document](https://skmtc.dev/seqbench/apis/seqbench-api/revisions/132575216865?raw)