---
title: "Submit a protein for domain/GO annotation"
method: POST
path: "/protein_annotate_submit"
tags: ["Proteins & peptides"]
---

# Submit a protein for domain/GO annotation

`POST /protein_annotate_submit`

## Request

- Base URL: `https://seqbench.com/api/v1`
- URL: `https://seqbench.com/api/v1/protein_annotate_submit`
- Auth: none required

Submit a protein sequence to EBI InterProScan for domain architecture, family and GO-term annotation, AND for membrane topology and signal-peptide prediction — EBI's default run includes Phobius, TMHMM and SignalP, so a transmembrane helix, its inside/outside topology and a cleaved signal peptide all come back without asking for them (see `topology` on the poll result). Returns a jobId immediately — the job itself takes minutes; poll it with protein_annotate_poll.

## Request body

- object
  - `sequence` string, required: Protein sequence, one-letter code (FASTA header, if any, is stripped).
  - `appl` string: Restrict to one member database (e.g. "PfamA"); omit to run EBI's defaults across all of them.
  - `goterms` boolean: Include GO-term cross-references.

## Response `200`

Tool ran successfully.

- SuccessResponse: Standard success envelope returned by every single-tool call.
  - `ok` unknown, required
  - `tool` string, required: The tool slug that ran.
  - `result` object, required: Tool-specific output object. Its shape depends on the tool — call `GET /{tool}` or see the docs for each tool's fields.
  - `gate` GateResult
    - `pass` boolean, required: True if and only if every hard check passed.
    - `checks` GateCheck[], required
      - `id` string, required
      - `label` string, required
      - `severity` 'hard' | 'soft', required
      - `pass` boolean, required
      - `message` string, required
      - `value` number
      - `threshold` string
    - `notChecked` string[], required: Honest list of what this gate does NOT verify.
  - `provenance` Provenance, required
    - `apiVersion` string, required
    - `tool` union, required: Tool slug, or an array of slugs (one per step) for a workflow.
      - string
      - string[]
    - `generatedAt` string, date-time, required: ISO 8601 timestamp of when the result was generated.

## Other responses

- `400`: Invalid argument or the tool could not run.
- `404`: Unknown tool.
- `429`: Rate limited (metered tools only). Body carries code `rate_limited` and retryable `true`; honor the Retry-After header.
- `503`: A metered tool is momentarily at capacity. The identical request should succeed after Retry-After; body carries code `upstream_unavailable`.

---

[API](https://skmtc.dev/seqbench/apis/seqbench-api.md) · [All operations](https://skmtc.dev/seqbench/apis/seqbench-api/llms.txt) · [OpenAPI document](https://skmtc.dev/seqbench/apis/seqbench-api/revisions/132575216865?raw)