---
title: "Protease Digestion"
method: POST
path: "/protease_digestion"
tags: ["Proteins & peptides"]
---

# Protease Digestion

`POST /protease_digestion`

## Request

- Base URL: `https://seqbench.com/api/v1`
- URL: `https://seqbench.com/api/v1/protease_digestion`
- Auth: none required

In-silico protease/chemical digestion: cleave a protein and report each peptide's position, length and neutral mass. CNBr masses assume terminal Met becomes homoserine lactone; the sequence retains M and the response labels the modification.

Batchable via `POST /batch`.

## Request body

- object
  - `sequence` string, required: Protein sequence (one-letter amino-acid codes; non-AA characters ignored).
  - `protease` 'trypsin' | 'lysc' | 'argc' | 'chymotrypsin' | 'gluc' | 'aspn' | 'cnbr': Protease or chemical cleavage agent.
  - `missedCleavages` 0 | 1 | 2: Allowed missed internal cleavages (0–2).
  - `minMass` number: Optional lower bound on neutral monoisotopic mass (Da).
  - `maxMass` number: Optional upper bound on neutral monoisotopic mass (Da).
  - `maxPeptides` integer: Cap on the number of returned peptides.

## Response `200`

Tool ran successfully.

- SuccessResponse: Standard success envelope returned by every single-tool call.
  - `ok` unknown, required
  - `tool` string, required: The tool slug that ran.
  - `result` object, required: Tool-specific output object. Its shape depends on the tool — call `GET /{tool}` or see the docs for each tool's fields.
  - `gate` GateResult
    - `pass` boolean, required: True if and only if every hard check passed.
    - `checks` GateCheck[], required
      - `id` string, required
      - `label` string, required
      - `severity` 'hard' | 'soft', required
      - `pass` boolean, required
      - `message` string, required
      - `value` number
      - `threshold` string
    - `notChecked` string[], required: Honest list of what this gate does NOT verify.
  - `provenance` Provenance, required
    - `apiVersion` string, required
    - `tool` union, required: Tool slug, or an array of slugs (one per step) for a workflow.
      - string
      - string[]
    - `generatedAt` string, date-time, required: ISO 8601 timestamp of when the result was generated.

## Other responses

- `400`: Invalid argument or the tool could not run.
- `404`: Unknown tool.
- `429`: Rate limited (metered tools only). Body carries code `rate_limited` and retryable `true`; honor the Retry-After header.
- `503`: A metered tool is momentarily at capacity. The identical request should succeed after Retry-After; body carries code `upstream_unavailable`.

---

[API](https://skmtc.dev/seqbench/apis/seqbench-api.md) · [All operations](https://skmtc.dev/seqbench/apis/seqbench-api/llms.txt) · [OpenAPI document](https://skmtc.dev/seqbench/apis/seqbench-api/revisions/132575216865?raw)