---
title: "Prime-editing efficiency (PRIDICT2.0)"
method: POST
path: "/prime_editing_efficiency"
tags: ["CRISPR"]
---

# Prime-editing efficiency (PRIDICT2.0)

`POST /prime_editing_efficiency`

## Request

- Base URL: `https://seqbench.com/api/v1`
- URL: `https://seqbench.com/api/v1/prime_editing_efficiency`
- Auth: none required

Predict per-pegRNA prime-editing efficiency for one edit with PRIDICT2.0, and return the top-scoring pegRNA designs ranked by it. Takes the target as context, the edit in brackets, then context — ACGT...(A/G)...ACGT, with roughly 100+ bp each side — and enumerates PBS/RTT length combinations, scoring every one in HEK293 and K562. Each candidate comes back with both scores, its percentile against the training library, its rank, the spacer, PBS and RTT lengths, the full pegRNA, and Golden Gate cloning oligos. Use it to CHOOSE between designs; the number is not a promised editing percentage.

## Request body

- object
  - `sequence` string, required: Target with the edit in brackets: context, then (original/edited), then context. Roughly 100+ bp each side — the model reads that context. Keep unchanged flanking bases OUTSIDE the brackets: T(a/g)C, not (TAC/TGC). Insertions and deletions leave one side empty, e.g. (/AGG) or (AGG/).
  - `cellType` 'HEK' | 'K562': Which trained context to RANK by. Both scores are always returned; this decides the ordering. There is no generic-mammalian option because the model has no such training data.
  - `topN` integer: How many top-ranked pegRNAs to return, out of the hundreds enumerated. Max 50.
  - `use5Folds` boolean: Average all five trained folds instead of the first. Modestly steadier scores for five times the compute, and it is charged five times as much.

## Response `200`

Tool ran successfully.

- SuccessResponse: Standard success envelope returned by every single-tool call.
  - `ok` unknown, required
  - `tool` string, required: The tool slug that ran.
  - `result` object, required: Tool-specific output object. Its shape depends on the tool — call `GET /{tool}` or see the docs for each tool's fields.
  - `gate` GateResult
    - `pass` boolean, required: True if and only if every hard check passed.
    - `checks` GateCheck[], required
      - `id` string, required
      - `label` string, required
      - `severity` 'hard' | 'soft', required
      - `pass` boolean, required
      - `message` string, required
      - `value` number
      - `threshold` string
    - `notChecked` string[], required: Honest list of what this gate does NOT verify.
  - `provenance` Provenance, required
    - `apiVersion` string, required
    - `tool` union, required: Tool slug, or an array of slugs (one per step) for a workflow.
      - string
      - string[]
    - `generatedAt` string, date-time, required: ISO 8601 timestamp of when the result was generated.

## Other responses

- `400`: Invalid argument or the tool could not run.
- `404`: Unknown tool.
- `429`: Rate limited (metered tools only). Body carries code `rate_limited` and retryable `true`; honor the Retry-After header.
- `503`: A metered tool is momentarily at capacity. The identical request should succeed after Retry-After; body carries code `upstream_unavailable`.

---

[API](https://skmtc.dev/seqbench/apis/seqbench-api.md) · [All operations](https://skmtc.dev/seqbench/apis/seqbench-api/llms.txt) · [OpenAPI document](https://skmtc.dev/seqbench/apis/seqbench-api/revisions/132575216865?raw)