---
title: "Deep plasmid annotation (pLannotate)"
method: POST
path: "/plasmid_deep_annotate"
tags: ["Enzymes & cloning"]
---

# Deep plasmid annotation (pLannotate)

`POST /plasmid_deep_annotate`

## Request

- Base URL: `https://seqbench.com/api/v1`
- URL: `https://seqbench.com/api/v1/plasmid_deep_annotate`
- Auth: none required

Annotate a plasmid against pLannotate's open-source feature library — a much larger signature set (GenoLIB parts + Swiss-Prot + FPbase + Rfam, cross-referenced against ~195k Addgene-deposited plasmids) than plasmid_annotate's built-in curated list, and it reports partial and low-identity hits as graded alignments rather than the pass/fail signature match plasmid_annotate does (that one is not exact-only either — signatures of 20 bp or more tolerate up to ~10% mismatches — but it reports a hit or nothing, with a `mismatches` count and an `exact` flag). Each feature here carries its percent identity, reference coverage and a fragment flag so you can judge a weak hit. Runs a multi-second search on a shared service and is therefore rate limited (see 429/503); use plasmid_annotate for an instant, unmetered first pass.

## Request body

- object
  - `sequence` string, required: Nucleotide sequence (raw or FASTA). A, C, G, T, N only — other IUPAC codes are rejected rather than silently dropped, because pLannotate's search engines discard them and every coordinate after would shift. Max 150,000 bp.
  - `circular` boolean: Treat the sequence as a circular plasmid (vs. linear).

## Response `200`

Tool ran successfully.

- SuccessResponse: Standard success envelope returned by every single-tool call.
  - `ok` unknown, required
  - `tool` string, required: The tool slug that ran.
  - `result` object, required: Tool-specific output object. Its shape depends on the tool — call `GET /{tool}` or see the docs for each tool's fields.
  - `gate` GateResult
    - `pass` boolean, required: True if and only if every hard check passed.
    - `checks` GateCheck[], required
      - `id` string, required
      - `label` string, required
      - `severity` 'hard' | 'soft', required
      - `pass` boolean, required
      - `message` string, required
      - `value` number
      - `threshold` string
    - `notChecked` string[], required: Honest list of what this gate does NOT verify.
  - `provenance` Provenance, required
    - `apiVersion` string, required
    - `tool` union, required: Tool slug, or an array of slugs (one per step) for a workflow.
      - string
      - string[]
    - `generatedAt` string, date-time, required: ISO 8601 timestamp of when the result was generated.

## Other responses

- `400`: Invalid argument or the tool could not run.
- `404`: Unknown tool.
- `429`: Rate limited (metered tools only). Body carries code `rate_limited` and retryable `true`; honor the Retry-After header.
- `503`: A metered tool is momentarily at capacity. The identical request should succeed after Retry-After; body carries code `upstream_unavailable`.

---

[API](https://skmtc.dev/seqbench/apis/seqbench-api.md) · [All operations](https://skmtc.dev/seqbench/apis/seqbench-api/llms.txt) · [OpenAPI document](https://skmtc.dev/seqbench/apis/seqbench-api/revisions/132575216865?raw)