---
title: "List AlignedNucleotideSequence items"
method: GET
path: "/api/v3/nucleotide-alignment/{nucleotide_alignment_id}/aligned-nucleotide-sequences/items"
tags: ["Nucleotide Alignment"]
---

# List AlignedNucleotideSequence items

`GET /api/v3/nucleotide-alignment/{nucleotide_alignment_id}/aligned-nucleotide-sequences/items`

## Request

- Base URL: `https://{tenant_name}.benchling.com`
- URL: `https://{tenant_name}.benchling.com/api/v3/nucleotide-alignment/{nucleotide_alignment_id}/aligned-nucleotide-sequences/items`
- Auth: HTTP bearer

List AlignedNucleotideSequence items.

## Path parameters

- `nucleotide_alignment_id` string, required

## Query parameters

- `nextToken` string
- `pageSize` integer

## Response `200`

OK

- AlignedNucleotideSequencePaginatedList
  - `items` AlignedNucleotideSequence[]
    - `__typename` string
    - `bases` string: The aligned bases for this row, including gap characters (-) for insertions and deletions relative to the reference sequence.
    - `id` string: The ID of the Aligned Nucleotide Sequence.
    - `name` string: The name of the Aligned Nucleotide Sequence.
    - `nucleotideAlignment` NucleotideAlignmentRef
      - `__typename` string
      - `id` string, api_id
    - `pairwiseIdentity` number, double: Fraction of bases between trimStart and trimEnd that match the reference sequence within the trimmed region.
    - `sequence` NucleotideSequenceRef
      - `__typename` string
      - `id` string, api_id
    - `trimEnd` integer: Exclusive end index into the gapped bases string for the region included in the alignment. Bases outside the trimmed region are excluded from the alignment and from the pairwise identity calculation. The trim region is determined by trace data, but can also be edited manually.
    - `trimStart` integer: Inclusive start index into the gapped bases string for the region included in the alignment. Bases outside the trimmed region are excluded from the alignment and from the pairwise identity calculation. The trim region is determined by trace data, but can also be edited manually.
  - `nextToken` string, nullable

## Other responses

- `400`: Bad Request
- `403`: Forbidden
- `404`: Not Found
- `429`: Too Many Requests
- `500`: Internal Server Error

---

[API](https://skmtc.dev/benchling/apis/benchling-api.md) - [All operations](https://skmtc.dev/benchling/apis/benchling-api/llms.txt) - [OpenAPI document](https://skmtc.dev/benchling/apis/benchling-api/revisions/c9f087b98b8b?raw)