---
title: "Get Enzyme by ID"
method: GET
path: "/api/v3/enzyme/{enzyme_id}"
tags: ["Enzyme"]
---

# Get Enzyme by ID

`GET /api/v3/enzyme/{enzyme_id}`

## Request

- Base URL: `https://{tenant_name}.benchling.com`
- URL: `https://{tenant_name}.benchling.com/api/v3/enzyme/{enzyme_id}`
- Auth: HTTP bearer

Get a single Enzyme by ID.

## Path parameters

- `enzyme_id` string, required

## Query parameters

- `returning` string[]
- `omit` string[]

## Headers

- `EARLY-ACCESS` string

## Response `200`

OK

- Enzyme: Represents a restriction enzyme from Benchling's global catalog of commercially available enzymes. Restriction enzymes (also known as restriction endonucleases) are molecular scissors that recognize specific DNA sequences and cleave the DNA at defined positions. Each enzyme has a recognition sequence (restrictionSiteBases), such as GAATTC for EcoRI, and cut site details (see CutSiteDetail) specifying where the enzyme cuts relative to that recognition site. Enzymes can produce blunt ends or sticky ends (overhangs) depending on their cut pattern. Users can organize frequently used enzymes into EnzymeLists for convenient access during cloning workflow design.
  - `__typename` string
  - `cutSites` CutSiteDetail[]: Either one (for single cutters) or two (for double cutters) cut details.
    - `__typename` string
    - `offset` integer: Location, relative to `position`, where the 3' cut is made.
    - `position` integer: Location, relative to the restriction site bases, where the 5' cut is made.
  - `id` string: Id of the Enzyme
  - `isoschizomers` string, uri: Other enzymes in the catalog that recognize the same restriction site.
  - `name` string: The name of the Enzyme, like EcoRI.
  - `restrictionSiteBases` string: The DNA bases recognized by the Enzyme.

## Other responses

- `400`: Bad Request
- `403`: Forbidden
- `404`: Not Found
- `429`: Too Many Requests
- `500`: Internal Server Error

---

[API](https://skmtc.dev/benchling/apis/benchling-api.md) - [All operations](https://skmtc.dev/benchling/apis/benchling-api/llms.txt) - [OpenAPI document](https://skmtc.dev/benchling/apis/benchling-api/revisions/c9f087b98b8b?raw)