--- title: "Create DnaSequence" method: POST path: "/api/v3/dna-sequence" tags: ["DNA Sequence"] --- # Create DnaSequence `POST /api/v3/dna-sequence` ## Request - Base URL: `https://{tenant_name}.benchling.com` - URL: `https://{tenant_name}.benchling.com/api/v3/dna-sequence` - Auth: HTTP bearer Create DnaSequence. ## Request body - CreateDnaSequenceInput - `aliases` string[]: Array of aliases - `annotations` NucleotideAnnotationInput[]: Annotations on the DNA sequence, not including translations or primers. Annotations on parts are not included. - `color` string: Hex color when displaying the annotation. A random color is assigned if omitted. - `customFields` CustomFieldInput[]: Custom fields on the annotation. Each field name may be at most 1024 characters and each value at most 100000 characters. - `name` string, required: Name of the custom field. - `value` string: Value of the custom field. Defaults to an empty string if omitted. - `end` integer, required: 0-based exclusive end index. For ranges ending at the sequence end, API responses return `sequenceLength`, never `0`. On input, `sequenceLength` or `0` may be used. - `name` string, required: Name of the annotation. - `notes` string: Text notes on the annotation. Defaults to an empty string if omitted. - `start` integer, required: 0-based inclusive start index. - `strand` 'FORWARD' | 'REVERSE' | 'STRANDLESS', required: Strand for this annotation; `FORWARD`, `REVERSE`, or `STRANDLESS`. - `type` string: Feature type of the annotation. - `authorIds` string[] - `bases` string, required: Bases of the forward strand of the DNA sequence. - `customFields` object: Custom fields set on the DNA sequence. - `entityRegistryId` string: Registry ID to assign to the entity. If one is specified, the labeling_strategy must be unspecified. - `folderId` string, required - `isCircular` boolean: Whether the sequence forms a closed loop, with the last base connecting back to the first. Defaults to false if not specified. - `name` string, required: Name of the item. - `namingStrategy` 'NEW_IDS' | 'IDS_FROM_NAMES' | 'DELETE_NAMES' | 'SET_FROM_NAME_PARTS' | 'REPLACE_NAMES_FROM_PARTS' | 'KEEP_NAMES' | 'REPLACE_ID_AND_NAME_FROM_PARTS': Strategy to use for labeling the item in the registry. If one is specified, the entity_registry_id must be unspecified. - `parts` NucleotidePartInput[]: Part features linking regions of this sequence to other nucleotide sequences. - `end` integer, required: 0-based exclusive end index. For ranges ending at the sequence end, API responses return `sequenceLength`, never `0`. On input, `sequenceLength` or `0` may be used. - `sequenceId` string, required: Nucleotide sequence this part refers to. - `start` integer, required: 0-based inclusive start index. - `strand` 'FORWARD' | 'REVERSE': Strand for this part; `FORWARD` or `REVERSE`. Defaults to `FORWARD` if omitted. - `primers` PrimerInput[]: Primer bind features on the DNA sequence. Primers on parts are not included. - `location` union, required - object: bind_and_overhang variant - `locationType` 'bind_and_overhang', required: Discriminator for primer binding location. Use `start_and_end` for start/end coordinates, or `bind_and_overhang` for bind position and overhang length. - `bindPosition` integer, required: 0-based index of the base at the 3' end of the binding region. - `overhangLength` integer, required: Number of 5' primer bases that are not part of the binding region. - object: start_and_end variant - `locationType` 'start_and_end', required: Discriminator for primer binding location. Use `start_and_end` for start/end coordinates, or `bind_and_overhang` for bind position and overhang length. - `end` integer, required: 0-based exclusive end of the binding region. For ranges ending at the sequence end, API responses return `sequenceLength`, never `0`. On input, `sequenceLength` or `0` may be used. - `start` integer, required: 0-based inclusive start of the binding region. - `oligoId` string, required: Linked Oligo entity, which holds the name, bases, and color for this primer bind site. - `strand` 'FORWARD' | 'REVERSE', required: Strand for this primer; `FORWARD` or `REVERSE`. - `registryId` string, nullable: A not null value means the entity should be registered. If not null, the value must match the registry of the item's schema. - `schemaFields` object - `schemaId` string - `translations` TranslationInput[]: Translation annotations present on the DNA sequence. Translations may be one region or split across multiple regions. Translations on parts are not included. - `color` string: Hex color when displaying the translation. Defaults to light blue (`#84B0DC`) if omitted. - `customFields` CustomFieldInput[]: Custom fields on the translation. - `name` string, required: Name of the custom field. - `value` string: Value of the custom field. Defaults to an empty string if omitted. - `geneticCode` 'STANDARD' | 'VERTEBRATE_MITOCHONDRIAL' | 'YEAST_MITOCHONDRIAL' | 'MOLD_PROTOZOAN_COELENTERATE_MITOCHONDRIAL_MYCOPLASMA_SPIROPLASMA' | 'INVERTEBRATE_MITOCHONDRIAL' | 'CILIATE_DASYCLADACEAN_HEXAMITA_NUCLEAR' | 'ECHINODERM_FLATWORM_MITOCHONDRIAL' | 'EUPLOTID_NUCLEAR' | 'BACTERIAL_ARCHAEAL_PLANT_PLASTID' | 'ALTERNATIVE_YEAST_NUCLEAR' | 'ASCIDIAN_MITOCHONDRIAL' | 'ALTERNATIVE_FLATWORM_MITOCHONDRIAL' | 'CHLOROPHYCEAN_MITOCHONDRIAL' | 'TREMATODE_MITOCHONDRIAL' | 'SCENEDESMUS_OBLIQUUS_MITOCHONDRIAL' | 'THRAUSTOCHYTRIUM_MITOCHONDRIAL' | 'RHABDOPLEURIDAE_MITOCHONDRIAL' | 'CANDIDATE_DIVISION_SR1_GRACILIBACTERIA' | 'PACHYSOLEN_TANNOPHILUS_NUCLEAR' | 'MESODINIUM_NUCLEAR' | 'PERITRICH_NUCLEAR' | 'CEPHALODISCIDAE_MITOCHONDRIAL_UAA_TYR': Genetic code for the translation. Defaults to `STANDARD` if omitted. Genetic codes with ambiguous stop codons are not supported: `BLASTOCRITHIDIA_NUCLEAR`, `CONDYLOSTOMA_NUCLEAR`, `KARYORELICT_NUCLEAR`. - `name` string: Name of the translation. - `notes` string: Text notes on the translation. Defaults to an empty string if omitted. - `regions` TranslationRegionInput[], required: Array of regions to translate. For single-region translations, provide one element. - `end` integer, required: 0-based exclusive end index. For ranges ending at the sequence end, API responses return `sequenceLength`, never `0`. On input, `sequenceLength` or `0` may be used. - `start` integer, required: 0-based inclusive start index. - `strand` 'FORWARD' | 'REVERSE', required: Strand for this translation; `FORWARD` or `REVERSE`. - `translateFirstCodonToMethionine` boolean: Whether or not the first codon has been forced to be translated as methionine. Defaults to false if omitted. ## Response `201` Created - DnaSequence: Represents a DNA sequence in Benchling's molecular biology platform. A `DnaSequence` stores the nucleotide bases of the forward strand along with rich annotations for biological features such as genes, promoters, and regulatory elements. Sequences can be linear or circular (controlled by `isCircular`) and support translation annotations for protein-coding regions (see `Translation`), primer binding sites (see `Primer`), and part features linking to other sequences (see `DnaPart`). DNA sequences can be registered in the Registry with a unique `entityRegistryId`, organized in folders, and linked to other entities via schema fields. Also known as "Sequence" in the Benchling interface, with IDs prefixed by 'seq'. - `__typename` string - `aliases` string[], nullable: Array of aliases - `annotations` string, uri: Annotations on the DNA sequence, not including translations or primers. Annotations on parts are not included. - `archiveReason` string, nullable - `archived` boolean - `bases` string: Bases of the forward strand of the DNA sequence. - `createdAt` string, datetime, nullable - `customFields` CustomField[]: Custom fields set on the DNA sequence. - `__typename` string - `name` string, nullable - `value` string, nullable - `entityRegistryId` string, nullable: The registry-assigned identifier for this item (e.g. its registry ID shown in the UI), present only once the item has been registered. Null if the item is not registered. - `folder` FolderRef - `__typename` string - `id` string, api_id - `id` string - `isCircular` boolean: Whether the DNA sequence is circular or linear. - `modifiedAt` string, datetime, nullable - `name` string: Name of the item. - `nucleotideAlignments` string, uri: Nucleotide alignments that use this sequence as the reference sequence. For template alignments, this is the template sequence; for consensus alignments, this is the derived consensus sequence. - `parts` string, uri: Part features linking regions of this sequence to other nucleotide sequences. - `primers` string, uri: Primer bind features on the DNA sequence. Primers on parts are not included. - `project` ProjectRef - `__typename` string - `id` string, api_id - `registrationOrigin` RegistrationOrigin: Captures the provenance of an entity's registration into the registry. The `originEntry` links to the `DocumentLike` (typically an entry or notebook) from which the entity was registered, providing traceability back to the original experimental context. The `registeredAt` timestamp records when the registration occurred. This information helps scientists understand where registered entities came from and when they were added to the registry. - `__typename` string - `originEntry` DocumentLikeRef - `__typename` string - `id` string, api_id - `registeredAt` string, datetime, nullable - `registry` RegistryRef - `__typename` string - `id` string, api_id - `schema` EntitySchemaRef - `__typename` string - `id` string, api_id - `startIndex` integer: Starting index for the sequence residues of linear DNA sequences. Implicitly 1 for other sequence types. - `translations` string, uri: Translation annotations present on the DNA sequence. Translations may be one region or split across multiple regions. Translations on parts are not included. - `typedSchema` DnaSequenceSchemaRef - `__typename` string - `id` string, api_id - `authors` string, uri, nullable - `creator` PrincipalRef - `__typename` string - `id` string, api_id - `schemaFields` SchemaFieldValue[], nullable - `__typename` string - `fieldDefinition` SchemaFieldDefinitionRef - `__typename` string - `id` string, api_id - `id` string - `linkedEntityId` string, nullable - `value` union: Types of values that Benchling can interpret. - object: A type that represents a list of BenchlingValues, used for multi-value cells (e.g. alias columns). - `__typename` 'ArrayValue', required: Discriminator value: ArrayValue - `value` SingularBenchlingValue[] - union: A union of value types that can appear as elements within an ArrayValue. Identical to BenchlingValue but excludes ArrayValue to prevent nesting. Keep in sync with BenchlingValue. - object: A type that represents boolean values. - … - object: A type that represents datetime values. - … - object: A type that represents date values. - … - object: A type that represents decimal value as strings. - … - object: A type that represents integer values. - … - object: A type that represents JSON values. - … - object: A type that represents a list of links to other objects. - … - object: A type that represents links to other objects. - … - object: A type that represents text (string) values with an associated URL. - … - object: A type that represents text (string) values. - … - object: A type that represents boolean values. - `__typename` 'BooleanValue', required: Discriminator value: BooleanValue - `value` boolean: The boolean value. - object: A type that represents datetime values. - `__typename` 'DateTimeValue', required: Discriminator value: DateTimeValue - `value` string, datetime: The datetime value with UTC as the timezone. - object: A type that represents date values. - `__typename` 'DateValue', required: Discriminator value: DateValue - `value` string, date: The date value. - object: A type that represents decimal value as strings. - `__typename` 'DecimalValue', required: Discriminator value: DecimalValue - `numericValue` number, double, nullable: Deprecated. The float representation of the decimal value. - `value` string, nullable: The decimal value in a string representation - object: A type that represents integer values. - `__typename` 'IntegerValue', required: Discriminator value: IntegerValue - `value` integer: The integer value. - object: A type that represents JSON values. - `__typename` 'JsonValue', required: Discriminator value: JsonValue - `value` union: The JSON value. - object - string[] - string - number, double - boolean - object: A type that represents a list of links to other objects. - `__typename` 'ObjectLinkListValue', required: Discriminator value: ObjectLinkListValue - `value` LinkableDomainObject[]: The list of objects that this value links to. Inaccessible objects may be returned instead if the object is not found with the current permission set. - union: A union representing items that can be linked to. If you have a type that can be linked to, it can only be added once it's in beta. - object: AaSequence variant - … - object: Attachment variant - … - object: Box variant - … - object: Container variant - … - object: CustomEntity variant - … - object: DnaOligo variant - … - object: DnaSequence variant - … - object: DropdownOption variant - … - object: Entry variant - … - object: Inaccessible variant - … - object: Location variant - … - object: MatrixPlate variant - … - object: Mixture variant - … - object: Molecule variant - … - object: OligoConjugate variant - … - object: OligoDuplex variant - … - object: Protein variant - … - object: Result variant - … - object: RnaOligo variant - … - object: RnaSequence variant - … - object: WellPlate variant - … - object: A type that represents links to other objects. - `__typename` 'ObjectLinkValue', required: Discriminator value: ObjectLinkValue - `value` union: A union representing items that can be linked to. If you have a type that can be linked to, it can only be added once it's in beta. - object: AaSequence variant - `__typename` 'AaSequence', required: Discriminator value: AaSequence - `id` string, api_id - object: Attachment variant - `__typename` 'Attachment', required: Discriminator value: Attachment - `id` string, api_id - object: Box variant - `__typename` 'Box', required: Discriminator value: Box - `id` string, api_id - object: Container variant - `__typename` 'Container', required: Discriminator value: Container - `id` string, api_id - object: CustomEntity variant - `__typename` 'CustomEntity', required: Discriminator value: CustomEntity - `id` string, api_id - object: DnaOligo variant - `__typename` 'DnaOligo', required: Discriminator value: DnaOligo - `id` string, api_id - object: DnaSequence variant - `__typename` 'DnaSequence', required: Discriminator value: DnaSequence - `id` string, api_id - object: DropdownOption variant - `__typename` 'DropdownOption', required: Discriminator value: DropdownOption - `id` string, api_id - object: Entry variant - `__typename` 'Entry', required: Discriminator value: Entry - `id` string, api_id - object: Inaccessible variant - `__typename` 'Inaccessible', required: Discriminator value: Inaccessible - `id` string, api_id - object: Location variant - `__typename` 'Location', required: Discriminator value: Location - `id` string, api_id - object: MatrixPlate variant - `__typename` 'MatrixPlate', required: Discriminator value: MatrixPlate - `id` string, api_id - object: Mixture variant - `__typename` 'Mixture', required: Discriminator value: Mixture - `id` string, api_id - object: Molecule variant - `__typename` 'Molecule', required: Discriminator value: Molecule - `id` string, api_id - object: OligoConjugate variant - `__typename` 'OligoConjugate', required: Discriminator value: OligoConjugate - `id` string, api_id - object: OligoDuplex variant - `__typename` 'OligoDuplex', required: Discriminator value: OligoDuplex - `id` string, api_id - object: Protein variant - `__typename` 'Protein', required: Discriminator value: Protein - `id` string, api_id - object: Result variant - `__typename` 'Result', required: Discriminator value: Result - `id` string, api_id - object: RnaOligo variant - `__typename` 'RnaOligo', required: Discriminator value: RnaOligo - `id` string, api_id - object: RnaSequence variant - `__typename` 'RnaSequence', required: Discriminator value: RnaSequence - `id` string, api_id - object: WellPlate variant - `__typename` 'WellPlate', required: Discriminator value: WellPlate - `id` string, api_id - object: A type that represents text (string) values with an associated URL. - `__typename` string, required - `url` string: The URL associated with the value. Please use `TextValue` if you don't want a URL. - `value` string: The text value. It may or may not be an empty string. - object: A type that represents text (string) values. - `__typename` string, required - `value` string: The text value. It may or may not be an empty string. ## Other responses - `400`: Bad Request - `403`: Forbidden - `404`: Not Found - `409`: Conflict - `429`: Too Many Requests - `500`: Internal Server Error ## Changes - **2026-11-05** `3873a10bf7b8`: 1 breaking, 1 info - added `subschema #1, subschema #8, subschema #9` to the `schemaFields/items/value/oneOf[subschema #1: BenchlingValue]/` response property `oneOf` list for the response status `201` - removed `subschema #1, subschema #8, subschema #9` from the `schemaFields/items/value/oneOf[subschema #1: BenchlingValue]/` response property `oneOf` list for the response status `201` [Operation history](https://skmtc.dev/benchling/apis/benchling-api/changes/api/v3/dna-sequence/post.md) --- [API](https://skmtc.dev/benchling/apis/benchling-api.md) - [All operations](https://skmtc.dev/benchling/apis/benchling-api/llms.txt) - [OpenAPI document](https://skmtc.dev/benchling/apis/benchling-api/revisions/3873a10bf7b8?raw)